Engineering bone-forming callus organoid implants in a xenogeneic-free differentiation medium

Engineering bone-forming callus organoid implants in a xenogeneic-free differentiation medium

Blog Article

The field of tissue engineering aspires to provide clinically relevant solutions for patients through the integration of developmental engineering principles with a bottom-up manufacturing approach.However, the manufacturing of cell-based Wooden Bus advanced therapy medicinal products is hampered by protocol complexity, lack of non-invasive critical quality controls, and dependency on animal-derived components for tissue differentiation.We investigate a serum-free, chemically defined, xeno- and lipid-free chondrogenic differentiation medium to generate bone-forming callus organoids.Our results show an increase in microtissue homogeneity during prolonged differentiation and the high quality of in vivo bone-forming organoids.

The low protein content of the culture medium potentially allows for the monitoring of relevant secreted biomarkers as (critical) quality attributes.Together, we envisage that this xeno- and lipid-free chondrogenic medium is compatible with industrial scale-up and Kids Hoodies automation while facilitating the implementation of non-invasive imaging and the use of quality control parameters based on secreted biomarkers.